Abstract: Aiming at Daqing crude oil blended with Russian crude oil from 5% to 10%, an industrial experiment on thixotropic characteristic of Daqing crude oil in TieDa Pipeline and Qingtie Pipeline is carried out. Experimental results display that Houska model can exactly describe thixotropic characteristic of Daqing crude oil under given measurement temperature and inlet temperature with multi-shear rate condition. Thixotropic model parameters of different measurement temperatures and inlet temperature under actual pipeline flow condition in TieDa pipeline and QinJing pipeline are obtained, which are of guiding significance in correctly calculating the pressure for pipeline shutting down and restarting.